ABSTRACT
The high incidence of cardiovascular diseases is a serious threat to human health, and endovascular surgery has become the standard treatment for most interventional cardiovascular diseases. The robotassisted endovascular surgery system further enhances surgeons' ability to perform minimally invasive endovascular procedures in interventional cardiology. This study presents a new robotic technique for coronary intervention from the perspective of clinical application. Aiming at clinical application scenarios, this scheme proposed an intuitive guide wire catheter mechanism design, which accurately and perfectly simulates the doctor's hand movements, realizes the positive and negative direction translation of the guide wire catheter, accurate torque control of the guide wire rotation and locking. The results of animal test showed that the R-OneTM has a high degree of dexterity, accuracy and stability,and meets the clinical needs.
Subject(s)
Animals , Cardiovascular Diseases , Catheterization , Equipment Design , Robotic Surgical Procedures , RoboticsABSTRACT
<p><b>OBJECTIVE</b>To investigate the correlation between expression of A-kinase anchoring protein 95 (AKAP95) and protein expression of cyclin E1 and cyclin D1 in lung cancer tissue.</p><p><b>METHODS</b>Fifty-one cases of lung cancer were included in the study. The protein expression of AKAP95, cyclin E1, and cyclin D1 were measured by immunohistochemistry.</p><p><b>RESULTS</b>The protein expression of cyclin E1 in lung cancer tissues was significantly higher than that in para-cancerous tissues (positive rate: 75.56%vs 20%, P < 0.01); its expression showed no relationship with histopathological type, lymph node metastasis, and cellular differentiation (P > 0.05). The protein expression of cyclin D1 in lung cancer tissues was higher than that in para-cancerous tissues (positive rate: 69.39% vs 14.29%); its expression showed a significant relationship with histopathological type (P < 0.05). The expression of AKAP95 was correlated with the protein expression of cyclin E1 and cyclin D1 in lung cancer tissues (P < 0.01).</p><p><b>CONCLUSION</b>Cyclin E1 and cyclin D1 are highly expressed in lung cancer tissue, suggesting that they play an important role in the development and progression of lung cancer. The protein expression of cyclin E1 has no relationship with cellular differentiation, lymph node metastasis, and histopathological type of lung cancer, and the protein expression of cyclin D1 has a significant relationship with histopathological type. The expression of AKAP95 is correlated with the protein expression of cyclin E1 and cyclin D1 in lung cancer tissue.</p>
Subject(s)
Adult , Aged , Humans , Middle Aged , A Kinase Anchor Proteins , Metabolism , Cyclin D1 , Metabolism , Cyclin E , Metabolism , Lung , Metabolism , Pathology , Lung Neoplasms , Metabolism , Pathology , Oncogene Proteins , MetabolismABSTRACT
Paraganglioma is a rare neuroendocrine neoplasm observed in patients of all ages, with an estimated incidence of 3/1,000,000 population. It has long been recognized that some cases are familial. The majority of these tumors are benign, and the only absolute criterion for malignancy is the presence of metastases at sites where chromaffin tissue is not usually found. Some tumors show gross local invasion and recurrence, which may indeed kill the patient, but this does not necessarily associate with metastatic potential. Here, we report a case of vertebral metastatic paraganglioma that occurred 19 months after the patient had undergone partial cystectomy for urinary bladder paraganglioma. We believe this to be a rarely reported bone metastasis of paraganglioma arising originally within the urinary bladder. In this report, we also provide a summary of the general characteristics of this disease, together with progress in diagnosis, treatment, and prognosis.
Subject(s)
Female , Humans , Middle Aged , Chromogranin A , Metabolism , Cystectomy , Paraganglioma , Metabolism , Pathology , General Surgery , Phosphopyruvate Hydratase , Metabolism , Prognosis , Radionuclide Imaging , Spinal Neoplasms , Diagnostic Imaging , General Surgery , Synaptophysin , Metabolism , Technetium , Thoracic Vertebrae , Tomography, X-Ray Computed , Urinary Bladder Neoplasms , Metabolism , Pathology , General SurgeryABSTRACT
<p><b>OBJECTIVE</b>To study the expression of connective tissue growth factor (CTGF) and its significance in sporadic ascending thoracic aortic aneurysm (AAA), and initially to investigate the mechanisms of pathological remodeling in AAA.</p><p><b>METHODS</b>AAA specimens were taken from 18 patients during elective surgical intervention, and 18 control specimens of ascending aorta were obtained from patients undergoing coronary artery bypass surgery. Specimens were stained with HE and Masson to evaluate the arrangement and aggregation of cells and collagen types I and III; immunohistochemistry staining was performed using antibodies directed against markers of CTGF; real-time PCR analysis was performed to quantify the expression level of CTGF and collagen types I and III.</p><p><b>RESULTS</b>Pathological results show degradation of elastin and hyperplasia of collagen fibers as well as disordered arrangement of smooth muscle cells in AAA. When compared with controls, protein levels of CTGF were significantly increased [(44 ± 4)% vs. (33 ± 5)%, P < 0.01]. Similar patterns were shown in mRNA levels of CTGF (P < 0.01). Using real-time PCR method, elevated levels (relative expression ratio of mRNA: 10.54/3.8 and 1.79/1.19, respectively; P < 0.01, both) of collagen types I and III were observed. CTGF expression had a correlation with both collagen fibers and aortic aneurysm diameter (r = 0.784, P < 0.01; r = 0.793, P < 0.01).</p><p><b>CONCLUSIONS</b>These results indicate increased expression of aortic collagen types I and III as well as CTGF in AAA specimens, which is likely to be responsible for the aortic wall pathological remodeling. The expression of CTGF was positively correlated with the aortic diameter. As a cytokines factor can stimulate collagen synthesis, CTGF may be involved in the pathogenesis and progression of AAA.</p>
Subject(s)
Aged , Female , Humans , Male , Middle Aged , Aorta , Metabolism , Pathology , Aortic Aneurysm, Thoracic , Metabolism , Pathology , Collagen Type I , Metabolism , Collagen Type III , Metabolism , Connective Tissue Growth Factor , MetabolismABSTRACT
Taking a typical-structure CT patient table as an example, methods of CAE applications to its design are introduced in detail in this paper, and theoretical analysis for the design result is made. This has provided a precise, efficient and reliable method for CT patient tables' mechanical designing.
Subject(s)
Equipment Design , Tomography, X-Ray ComputedABSTRACT
<p><b>OBJECTIVE</b>To analyze the natural change rule of active components of E. purpuea by measuring content of cichoric acid.</p><p><b>METHOD</b>Reverse HPLC method was used.</p><p><b>RESULT</b>The maximum cichoric acid content of the roots occured in seedling age of May, and that of the flowers occured in blooming stage of mid July, but cichoric acid in stems was generally low anyway. The maximum content of cichoric acid in the plant above ground occured in the blooming stage of mid July.</p><p><b>CONCLUSION</b>The measuring method of content of cichoric acid is successful and reliable. The optimum stage of harvest in Echinacea purpuea should be guided by natural change rule of cichoric acid content.</p>